PICK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PICK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 10983-2-AP

Synonyme

Protein kinase C-alpha-binding protein, PRKCABP, PRKCA-binding protein, PRKCA binding protein, PICK

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Galerie der Validierungsdaten

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  WB analysis using 10983-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10983-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10983-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10983-2-AP

  IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human stomach cancer using 10983-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10983-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10983-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10983-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PICK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PICK1 fusion protein Ag1443
• Vollständiger Name: protein interacting with PRKCA 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC017561
• Gene symbol: PICK1
• Gene ID (NCBI): 9463
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Res; PICK1 promotes caveolin-dependent degradation of TGF-β type I receptor.; Authors - Zhao Bing B ; KO Validated
mouse	WB	Environ Int; Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles.; Authors - Lixiao Zhou
mouse	IF	Front Neuroanat; Deletion of KIBRA, protein expressed in kidney and brain, increases filopodial-like long dendritic spines in neocortical and hippocampal neurons in vivo and in vitro.; Authors - Anja Blanque
human	WB,IHC	Cancer Sci; Protein interacting with C alpha kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer.; Authors - Zhang Bin B
human		Acta Pharm; Inhibitory effect of taspine derivative TAD1822-7 on tumor cell growth and angiogenesis via suppression of EphrinB2 and related signaling pathways.; Authors - Rui Liu
human	WB	Phytomedicine; Berberine inhibits the proliferation and migration of breast cancer ZR-75-30 cells by targeting Ephrin-B2.; Authors - Weina Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ambra (Verified Customer) (01-20-2021)

I used this rabbit anti-Pick 1:1000 in 5% milk in TBS-T on lysates from human brain (temporal cortex).NOTE: 1 clear band is visible using a 15% acrylamide gel. Lower gel percentage (10%) give a duplet band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue

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