PHKB Polyklonaler Antikörper

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• KD/KO Validated

PHKB Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13400-1-AP

Synonyme

PHKB, phosphorylase kinase, beta

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13400-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13400-1-AP

  IP result of anti-PHKB (IP:13400-1-AP, 4ug; Detection:13400-1-AP 1:500) with mouse heart tissue lysate 1680 ug.

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  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

13400-1-AP bindet in WB, IP, IHC, ELISA PHKB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHKB fusion protein Ag4240
• Vollständiger Name: phosphorylase kinase, beta
• Berechnetes Molekulargewicht: 1086 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC033657
• Gene symbol: PHKB
• Gene ID (NCBI): 5257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.

Publikationen

Species	Application	Title
mouse	IP, WB	Mol Endocrinol; 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.; Authors - Lai Guangrui G
mouse	WB	iScience; Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.; Authors - Motoyasu Hosokawa
human	WB,IHC	J Cancer Res Clin Oncol; Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker.; Authors - Guanghui Wang ; KD Validated