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PD-L1/CD274 Monoklonaler Antikörper
PD-L1/CD274 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IP, IHC, IF, FC, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2B11D11
Kat-Nr. : 66248-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A375-Zellen, A549-Zellen, Hausschwein-Lungengewebe, HepG2-Zellen, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe, K-562-Zellen, RAW 264.7-Zellen, THP-1-Zellen |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe, humanes Herzgewebe, humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe, Mausherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HeLa-Zellen |
| Erfolgreiche Detektion in FC | MDA-MB-231-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunhistochemie (IHC) | IHC : 1:5000-1:20000 |
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 5 publications below |
| WB | See 153 publications below |
| IHC | See 105 publications below |
| IF | See 54 publications below |
| IP | See 2 publications below |
| FC | See 3 publications below |
| CoIP | See 3 publications below |
Produktinformation
66248-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA PD-L1/CD274 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | PD-L1/CD274 fusion protein Ag12443 |
| Vollständiger Name | CD274 molecule |
| Berechnetes Molekulargewicht | 290 aa, 33 kDa |
| Beobachtetes Molekulargewicht | 45-50 kDa |
| GenBank-Zugangsnummer | BC074984 |
| Gene symbol | CD274 |
| Gene ID (NCBI) | 29126 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.
What is the molecular weight of PD-L1?
Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa).
What are the isoforms of PD-L1?
According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions.
What is the subcellular localization and tissue specificity of PD-L1?
It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues.
What is the function of PD-L1 in immune responses?
PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).
How can PD-L1's implication in cancer be used as a drug target?
In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for PD-L1/CD274 antibody 66248-1-Ig | Protokoll herunterladen |
| IHC protocol for PD-L1/CD274 antibody 66248-1-Ig | Protokoll herunterladen |
| IF protocol for PD-L1/CD274 antibody 66248-1-Ig | Protokoll herunterladen |
| FC protocol for PD-L1/CD274 antibody 66248-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Res Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria | ||
Cancer Cell ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis. | ||
Cell Metab NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. | ||
ACS Nano Self-Assembly of Podophyllotoxin-Loaded Lipid Bilayer Nanoparticles for Highly Effective Chemotherapy and Immunotherapy via Downregulation of Programmed Cell Death Ligand 1 Production. | ||
ACS Nano Mitochondria-Targeting Polymer Micelle of Dichloroacetate Induced Pyroptosis to Enhance Osteosarcoma Immunotherapy. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH hala (Verified Customer) (01-27-2023) | there is 2 bands one at the specific size and the other one is higher
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FH Emma (Verified Customer) (11-29-2021) | Used for IF on FFPE prostate tissue at 1:50 with Tris-EDTA antigen retrieval (pH 9.0).
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FH Marina (Verified Customer) (06-14-2021) | HCC1937 breast cancer cells were fixed with 4% PFA, permeabilized and stained overnight at 4ºC with anti-PD-L1 Ig diluted 1:300. Nuclei were stained with DAPI.
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