NRG1, isoform SMDF Polyklonaler Antikörper

NRG1, isoform SMDF Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10527-1-AP

Synonyme

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, NRG1, NRG1 isoform SMDF, NRG1, isoform SMDF, Pro NRG1, SMDF

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 10527-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10527-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 1x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10527-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe, humanes Magengewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

10527-1-AP bindet in WB, IHC, IF, ELISA NRG1, isoform SMDF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Vollständiger Name: neuregulin 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007675
• Gene symbol: NRG1
• Gene ID (NCBI): 3084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The antiiody is specific to NRG1 isoform SMDF.

Publikationen

Species	Application	Title
rat	WB	ACS Biomater Sci Eng; Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves; Authors - Lei Zhang
mouse	WB,IHC,IF	Ecotoxicol Environ Saf; Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.; Authors - Dongmin Yu
human	IHC	J Exp Clin Cancer Res; miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.; Authors - Dong-Min Shi
mouse	WB	Am J Physiol Renal Physiol; ErbB4 deletion accelerates renal fibrosis following renal injury.; Authors - Fenghua Zeng
human	IF	Folia Neuropathol; miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1.; Authors - Chengyan Sun
mouse	WB	Evid Based Complement Alternat Med; Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1.; Authors - Yuting Zhou