NPTX1 Polyklonaler Antikörper

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• KD/KO Validated

NPTX1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20656-1-AP

Synonyme

Neuronal pentraxin 1, neuronal pentraxin I, NPTX1

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Galerie der Validierungsdaten

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  WB analysis using 20656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20656-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20656-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 20656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NPTX1 antibody (20656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 20656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NPTX1 antibody (20656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20656-1-AP

  Immunofluorescent analysis of Hela cells, using NPTX1 antibody 20656-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, Maus-Cerebellum-Gewebe, Mauslebergewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

20656-1-AP bindet in WB, IHC, IF, CoIP, ELISA NPTX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: neuronal pentraxin I
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: NM_002522
• Gene symbol: NPTX1
• Gene ID (NCBI): 4884
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.

Publikationen

Species	Application	Title
mouse	WB, CoIP	Front Immunol; Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.; Authors - Réka Á Kovács
	WB	Acta Biochim Biophys Sin (Shanghai); miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells.; Authors - Kai Zhang
mouse	IF	PLoS One; RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis.; Authors - Brianna Dufek
human	WB	Cancer Manag Res; NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.; Authors - Hongfei Yan ; KD Validated
human	WB	PLoS One; Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis; Authors - Haijun Zhu ; KD Validated
mouse	WB	Oncogene; Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263; Authors - Lingyan Wang

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