NPHS2 Polyklonaler Antikörper

NPHS2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Kat-Nr. : 20384-1-AP

Synonyme

NPHS2, PDCN, Podocin, SRN1

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Galerie der Validierungsdaten

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  WB analysis of rat kidney using 20384-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 20384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of zebrafish embryos using 20384-1-AP

  The podocin antibody 20384-1-AP recognized the zebrafish pronephric glomeruli in zebrafish embryos (72 hour-old fish). IF result from Dr. Weibin Zhou.

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  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe, Zebrafischembryos

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 6 publications below
• IF: See 22 publications below

Produktinformation

20384-1-AP bindet in WB, IHC, IF, ELISA NPHS2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPHS2 fusion protein Ag14213
• Vollständiger Name: nephrosis 2, idiopathic, steroid-resistant (podocin)
• Berechnetes Molekulargewicht: 383 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC029141
• Gene symbol: NPHS2
• Gene ID (NCBI): 7827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)

Publikationen

Species	Application	Title
human	WB,IF	Kidney Int; A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome; Authors - Valeryia Kuzmuk
mouse	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
rat	IF,WB	Front Immunol; Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy; Authors - Xin Lv
mouse	IF	Aging (Albany NY); MiR-320a induces diabetic nephropathy via inhibiting MafB.; Authors - Mengying He
	WB	Cell Physiol Biochem; Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage.; Authors - Zhi Zuo
zebrafish	IF	Sci Rep; Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae.; Authors - Florian Siegerist

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