MPV17 Polyklonaler Antikörper

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• KD/KO Validated

MPV17 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10310-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis using 10310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10310-1-AP (MPV17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10310-1-AP

  WB result of MPV17 antibody (10310-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPV17 transfected HeLa cells.

• 

  IF Staining of HepG2 using 10310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10310-1-AP (MPV17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 3 publications below

Produktinformation

10310-1-AP bindet in WB, IF/ICC, ELISA MPV17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPV17 fusion protein Ag0312
• Vollständiger Name: MpV17 mitochondrial inner membrane protein
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC001115
• Gene symbol: MPV17
• Gene ID (NCBI): 4358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPV17, also termed as SYM1, encodes an inner mitochondrial membrane protein. It's potential candidate that may account for the mitochondrial (mt) DNA depletion syndromes (MDDS) characterized by a severe, tissue-specific decrease of mtDNA copy number and ultimate organ failure. It's a human ortholog of the mouse kidney disease gene Mpv17. Absence or malfunction of MPV17 causes oxidative phosphorylation (OXPHOS) failure and mtDNA depletion, not only in affected individuals but also in Mpv17-/- mice. MPV17 contribute to ROS homeostasis, but the downstream signaling is unknown yet. Catalog# 10310-1-AP is a rabbit polyclonal antibody raised against the full-length of human MPV17.

Publikationen

Species	Application	Title
human	WB,IF	Nat Genet; MPV17 encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion.; Authors - Spinazzola Antonella A ; KO Validated
mouse	WB	Nucleic Acids Res; Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model.; Authors - Chloe F Moss
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
mouse	WB	Mol Ther; AAV-mediated liver-specific MPV17 expression restores mtDNA levels and prevents diet-induced liver failure.; Authors - Emanuela Bottani ; KO Validated
mouse	WB	PLoS Genet; MPV17 Loss Causes Deoxynucleotide Insufficiency and Slow DNA Replication in Mitochondria.; Authors - Ilaria Dalla Rosa ; KO Validated
mouse	WB,IF	Clin Sci (Lond); Mitochondrial protein MPV17 promotes β-cell apoptosis in diabetogenesis; Authors - Qiaoli Tang

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