MMP13 Polyklonaler Antikörper

MMP13 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

362

Rezensionen (2)

Kat-Nr. : 18165-1-AP

Synonyme

CLG3, Matrix metalloproteinase 13, MMP 13, MMP13

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Galerie der Validierungsdaten

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  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 18165-1-AP

  IP result of anti-MMP13 (IP:18165-1-AP, 4ug; Detection:18165-1-AP 1:300) with MCF-7 cells lysate 2480 ug.

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  IHC staining of human hepatocirrhosis using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 18165-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, MCF-7-Zellen, MDA-MB-453s-Zellen, Rattenlebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe, humanes Mammakarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 264 publications below
• IHC: See 133 publications below
• IF: See 89 publications below

Produktinformation

18165-1-AP bindet in WB, IP, IHC, IF, ELISA MMP13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP13 fusion protein Ag12653
• Vollständiger Name: matrix metallopeptidase 13
• Berechnetes Molekulargewicht: 471 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC074808
• Gene symbol: MMP13
• Gene ID (NCBI): 4322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Publikationen

Species	Application	Title
human,mouse	IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human,mouse	WB,IF	Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
mouse,rat	WB,IF	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao
mouse	WB,IHC	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
mouse	IF	Nat Commun; Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing.; Authors - Kishor K Sivaraj
human	IF	Bone Res; Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.; Authors - Dong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xie (Verified Customer) (05-13-2021)

Good IHC staining for Rat MMP13.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: rat knee joints

MMP13 Antibody Immunohistochemistry validation (1:100 dilution) in rat knee joints (Cat no:18165-1-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

clear wb band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP13 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:18165-1-AP)

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