Integrin beta-6-Specific Polyklonaler Antikörper

Integrin beta-6-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19695-1-AP

Synonyme

Integrin beta 6, Integrin beta-6-Specific, integrin, beta 6, ITGB6

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Galerie der Validierungsdaten

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  WB analysis of A549 using 19695-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 19695-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 19695-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 19695-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 19695-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 19695-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 19695-1-AP (Integrin beta-6-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

19695-1-AP bindet in WB, IP, IHC, IF, ELISA Integrin beta-6-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: integrin, beta 6
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: NM_000888
• Gene symbol: ITGB6
• Gene ID (NCBI): 3694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. The antibody is specific to ITGB6.

Publikationen

Species	Application	Title
rat,mouse	WB	Glia; αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.; Authors - Kathleen K Catignas
human	IHC	Cell Biosci; Protein expression of eIF4E and integrin αvβ6 in colon cancer can predict clinical significance, reveal their correlation and imply possible mechanism of interaction.; Authors - Zhengchuan Niu
human	WB,IF,IP	J Exp Clin Cancer Res; Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.; Authors - Feikai Lin
mouse	IHC,IF	Mol Cell Endocrinol; Integrin β6 mediates epithelial-mesenchymal transition in diabetic kidney disease; Authors - Xiaoyu Li
human	WB,IHC	Adv Clin Exp Med; Upregulation of ITGB6 in primary palmar hyperhidrosis; Authors - Jian-Bo Lin
human	WB	Nat Commun; A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability; Authors - Ke Li

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