IRF2BP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IRF2BP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 18847-1-AP

Synonyme

IRF 2 binding protein 2, IRF 2BP2, IRF2BP2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 18847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18847-1-AP

  WB result of IRF2BP2 antibody (18847-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF2BP2 transfected HeLa cells.

• 

  WB analysis of HepG2 using 18847-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18847-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18847-1-AP

  IP Result of anti-IRF2BP2 (IP:18847-1-AP, 3ug; Detection:18847-1-AP 1:500) with K-562 cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18847-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

18847-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA IRF2BP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: interferon regulatory factor 2 binding protein 2
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: NM_182972
• Gene symbol: IRF2BP2
• Gene ID (NCBI): 359948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.

Publikationen

Species	Application	Title
human	WB	Cancer Discov; Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.; Authors - Jana M Ellegast ; KO Validated
		Hepatology; The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.; Authors - Xue Feng
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
mouse	WB,IHC	Hypertension; Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).; Authors - Jing Fang ; KO Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher

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