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IL-1R2 Monoklonaler Antikörper

IL-1R2 Monoklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H4H4

Kat-Nr. : 60262-1-Ig

Synonyme

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1 Receptor 2, IL-1 Receptor 2, IL1 Receptor II, IL-1 Receptor II, IL1R2, IL-1R2, IL1RB, IL-1RII, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    WB analysis of HeLa using 60262-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    IF Staining of HeLa using 60262-1-Ig

    Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below
IFSee 1 publications below

Produktinformation

60262-1-Ig bindet in WB, IF, ELISA IL-1R2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte ReaktivitätMaus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-1R2 fusion protein Ag4495
Vollständiger Name interleukin 1 receptor, type II
Berechnetes Molekulargewicht 398 aa, 45 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC039031
Gene symbol IL1R2
Gene ID (NCBI) 7850
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

Protokolle

Produktspezifische Protokolle
WB protocol for IL-1R2 antibody 60262-1-IgProtokoll herunterladen
IF protocol for IL-1R2 antibody 60262-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

Mater Today Bio

Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis

Authors - Jingzhe Wang
View Article
mouseIF,WB

Biochem Pharmacol

Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation

Authors - Meng-Bing Chen
View Article