IL-13RA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IL-13RA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11059-1-AP

Synonyme

CD213A2, CT19, IL 13 receptor subunit alpha 2, IL 13R, IL 13R alpha 2, IL 13R subunit alpha 2, IL 13RA2, IL13BP, IL13R, IL13RA2, IL-13RA2, IL13Ralpha2, Interleukin 13 binding protein

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Galerie der Validierungsdaten

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  WB analysis using 11059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL-13RA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 11059-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11059-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11059-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11059-1-AP (IL13RA2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11059-1-AP (IL13RA2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11059-1-AP (IL-13RA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11059-1-AP (IL-13RA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Maushodengewebe, Rattenlebergewebe, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe, humanes Hirngewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

11059-1-AP bindet in WB, IP, IF, IHC, ELISA IL-13RA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-13RA2 fusion protein Ag1534
• Vollständiger Name: interleukin 13 receptor, alpha 2
• Berechnetes Molekulargewicht: 380 aa, 44 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC020739
• Gene symbol: IL13RA2
• Gene ID (NCBI): 3598
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL13RA2, also named as IL13R and CD213a2, belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). IL13RA2 lacks the cytoplasmic domain for signaling, indicating that it acts as a decoy receptor. IL13RA2 gene polymorphisms have been associated with systemic sclerosis (PMID: 16981293). Overexpression of IL13RA2 gene has been reported in several types of cancer, including melanoma, lung cancer, and brain tumor, which makes it a potential immunotherapeutic target (PMID: 19895199; 24970476; 24021875).

Publikationen

Species	Application	Title
human	IHC	Neuro Oncol; Correction to: Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma; Authors -
rat	IF	J Control Release; In situ targeting nanoparticles-hydrogel hybrid system for combined chemo-immunotherapy of glioma.; Authors - Xiaoqi Wang
human	WB,IF,IP	Cancer Biol Med; IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation.; Authors - Tingting Feng ; KD Validated
human	IF	Front Neurosci; Outer brain barriers in rat and human development.; Authors - Christian B Brøchner
human	WB	Front Pharmacol; Isorhamnetin Enhances the Radiosensitivity of A549 Cells Through Interleukin-13 and the NF-κB Signaling Pathway.; Authors - Yarong Du
human,mouse	WB,IHC	Front Physiol; Downregulation of Interleukin-13 Receptor α2 Inhibits Angiogenic Formation Mediated by Chitinase 3-Like 1 in Late Atherosclerotic Lesions of apoE-/- Mice.; Authors - Qi Xue ; KD Validated

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