IKBKG Polyklonaler Antikörper

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• KD/KO Validated

IKBKG Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 18474-1-AP

Synonyme

AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator

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Galerie der Validierungsdaten

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  WB analysis using 18474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP (IKBKG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP (IKBKG antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse embryo using 18474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18474-1-AP

  Immunofluorescent analysis of HeLa cells using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Mausembryogewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

18474-1-AP bindet in WB, IP, IHC, IF, ELISA IKBKG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IKBKG fusion protein Ag13358
• Vollständiger Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC012114
• Gene symbol: IKBKG
• Gene ID (NCBI): 8517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1.; Authors - Xinyue Huang
human	WB	Cell Death Differ; IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.; Authors - Jia C-H CH ; KD Validated
human	WB	Mol Cell Proteomics; Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS; Authors - Xiurong Wu
human	WB	Mol Oncol; RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages.; Authors - Zhenyu Yao
human	WB,IF	FASEB J; 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.; Authors - Stefanie Inglis
human	WB	Cell Mol Immunol; PMID: 28392573; Authors - Yushun Wan

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