HOMER2 Polyklonaler Antikörper

HOMER2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11143-1-AP

Synonyme

Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD

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  WB analysis using 11143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 11143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 11143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 11143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HOMER2 antibody (11143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen, LNCaP-Zellen, MCF-7-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11143-1-AP bindet in WB, IHC, IF/ICC, ELISA HOMER2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOMER2 fusion protein Ag1606
• Vollständiger Name: homer homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC012109
• Gene symbol: HOMER2
• Gene ID (NCBI): 9455
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin