HLA-DMA Polyklonaler Antikörper

HLA-DMA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11838-1-AP

Synonyme

D6S222E, DAMA 147C13.1 001, DAMC 226H3.5 001, DAQB 96B10.1 001, DASS 67H14.8 001, DMA, DMA protein, hCG_2001572, HLA DMA, HLA DMA protein, HLADM, HLA-DMA, RING6, XXbac BCX136A3.1 001, XXbac BPG181M17.3 001

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Galerie der Validierungsdaten

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  WB analysis of rat spleen using 11838-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11838-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11838-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11838-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenmilzgewebe, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11838-1-AP bindet in WB, IHC, ELISA HLA-DMA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA-DMA fusion protein Ag2452
• Vollständiger Name: major histocompatibility complex, class II, DM alpha
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC026279
• Gene symbol: HLA-DMA
• Gene ID (NCBI): 3108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides. This antibody recognizes the 33kd HLA-DMA and the 50-60kd dimer in Western blotting.