GSNOR,ADH5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Kat-Nr. : 11051-1-AP

Synonyme

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

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Galerie der Validierungsdaten

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  WB analysis using 11051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11051-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11051-1-AP

  IP result of anti-GSNOR,ADH5 (IP:11051-1-AP, 4ug; Detection:11051-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11051-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 11051-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human GSNOR,ADH5 (11051-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

11051-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA GSNOR,ADH5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSNOR,ADH5 fusion protein Ag1533
• Vollständiger Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC070491
• Gene symbol: ADH5
• Gene ID (NCBI): 128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publikationen

Species	Application	Title
human	WB	Blood; Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.; Authors - Anfeng Mu ; KO Validated
human	WB	Nat Commun; Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells; Authors - Akiko Tomita
human,mouse	WB,IHC	Mol Cell; Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.; Authors - Felix A Dingler ; KO Validated
mouse	WB	Cancer Res; S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase.; Authors - Salvatore Rizza ; KO Validated
mouse	WB	Antioxid Redox Signal; GSNOR deficiency enhances in situ skeletal muscle strength, fatigue resistance and RyR1 S-nitrosylation without impacting mitochondrial content and activity.; Authors - Younghye Moon ; KO Validated
mouse	WB	Redox Biol; GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.; Authors - Jing Li ; KD Validated

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