Gelsolin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Gelsolin Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11644-2-AP

Synonyme

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

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Galerie der Validierungsdaten

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  WB analysis using 11644-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11644-2-AP (Gelsolin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 11644-2-AP

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 11644-2-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of U2OS using 11644-2-AP

  U2OS lysates prepared and IP of GSN performed using 1.0 μg of 11644-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11644-2-AP (GSN antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, U2OS-Zellen
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

11644-2-AP bindet in WB, IP, IF, IHC, ELISA Gelsolin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gelsolin fusion protein Ag2239
• Vollständiger Name: gelsolin (amyloidosis, Finnish type)
• Berechnetes Molekulargewicht: 782 aa, 86 kDa
• Beobachtetes Molekulargewicht: 87-93 kDa
• GenBank-Zugangsnummer: BC026033
• Gene symbol: Gelsolin
• Gene ID (NCBI): 2934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).

Publikationen

Species	Application	Title
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB,IF	Am J Cancer Res; 3'untranslated regions (3'UTR) of Gelsolin mRNA displays anticancer effects in non-small cell lung cancer (NSCLC) cells.; Authors - Xiaohui Liu
human	WB, IHC	Gene; Role of gelsolin in cell proliferation and invasion of human hepatocellular carcinoma cells.; Authors - Biao Deng
mouse	WB	Biomed Pharmacother; RACK1 promotes the proliferation, migration and invasion capacity of mouse hepatocellular carcinoma cell line in vitro probably by PI3K/Rac1 signaling pathway.; Authors - Wu Jun J
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao