GAP43 Polyklonaler Antikörper

GAP43 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16971-1-AP

Synonyme

Axonal membrane protein GAP 43, B 50, GAP43, growth associated protein 43, Neural phosphoprotein B 50, Neuromodulin, PP46



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe, Rattenlungengewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16971-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GAP43 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, hamster, Hund, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GAP43 fusion protein Ag9294
Vollständiger Name growth associated protein 43
Berechnetes Molekulargewicht 238 aa, 25 kDa
Beobachtetes Molekulargewicht 43 kDa
GenBank-ZugangsnummerBC007936
Gene symbol GAP43
Gene ID (NCBI) 2596
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The neuronal growth-associated protein GAP43 is also known as neuromodulin, B-50, P-57, F1 and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration, and may contribute to presynaptic membrane changes in the adult, leading to the neurotransmitter release, endocytosis and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicated molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

Protokolle

Produktspezifische Protokolle
WB protocol for GAP43 antibody 16971-1-APProtokoll herunterladen
IHC protocol for GAP43 antibody 16971-1-APProtokoll herunterladen
IF protocol for GAP43 antibody 16971-1-APProtokoll herunterladen
IP protocol for GAP43 antibody 16971-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

Adv Sci (Weinh)

An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.

Authors - Jing Liu
ratWB

Mater Today Bio

Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury

Authors - Fangguo Liang
ratWB

Phytomedicine

Ginsenoside Rb1 protects hippocampal neurons in depressed rats based on mitophagy-regulated astrocytic pyroptosis

Authors - Yannan Li
ratWB

Environ Pollut

Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.

Authors - Rundong Liu
ratWB

Aging (Albany NY)

Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.

Authors - Xin Zhang
mouseWB

Sci Total Environ

Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.

Authors - Huanliang Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/500 GAP43. Very strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Human primary cortical neurons
GAP43 Antibody Immunohistochemistry validation (1/500 dilution) in Human primary cortical neurons (Cat no:16971-1-AP)