GABRA4 Polyklonaler Antikörper

GABRA4 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12979-1-AP

Synonyme

GABA A Receptor alpha 4, GABAA Receptor alpha 4, GABRA4

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 12979-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12979-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12979-1-AP

  IP result of anti-GABRA4 (IP:12979-1-AP, 4ug; Detection:12979-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Nierengewebe, Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

12979-1-AP bindet in WB, IP, ELISA GABRA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABRA4 fusion protein Ag3600
• Vollständiger Name: gamma-aminobutyric acid (GABA) A receptor, alpha 4
• Berechnetes Molekulargewicht: 554 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62-70 kDa
• GenBank-Zugangsnummer: BC035055
• Gene symbol: GABRA4
• Gene ID (NCBI): 2557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Electro-acupuncture alleviates adolescent cocaine exposure-enhanced anxiety-like behaviors in adult mice by attenuating the activities of PV interneurons in PrL.; Authors - Jiaxun Nie
human	IP,WB	Cell Mol Life Sci; Cyclin D1-Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors; Authors - Neus Pedraza
human	WB	Genes Dis; The Traf2 and NcK interacting kinase inhibitor NCB-0846 suppresses seizure activity involving the decrease of GRIA1; Authors - Min Wang

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