FICD Polyklonaler Antikörper

FICD Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11974-1-AP

Synonyme

AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041

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Galerie der Validierungsdaten

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  WB analysis of A549 using 11974-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11974-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11974-1-AP bindet in WB, IHC, ELISA FICD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FICD fusion protein Ag2597
• Vollständiger Name: FIC domain containing
• Berechnetes Molekulargewicht: 458 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001342
• Gene symbol: FICD
• Gene ID (NCBI): 11153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenosine monophosphate-protein transferase FICD, also named as HIP13 or HYPE, is a 458 amino acid protein, which contains one fido domain and two TPR repeats. FICD localizes in the membrane and belongs to the fic family. FICD as an adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. The fido domain mediates the adenylyltransferase activity