FBXL14 Polyklonaler Antikörper

FBXL14 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13934-1-AP

Synonyme

F box/LRR repeat protein 14, Fbl14, FBXL14

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13934-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13934-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13934-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13934-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 13934-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13934-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13934-1-AP bindet in WB, IHC, IF, ELISA FBXL14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXL14 fusion protein Ag4975
• Vollständiger Name: F-box and leucine-rich repeat protein 14
• Berechnetes Molekulargewicht: 418 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC028132
• Gene symbol: FBXL14
• Gene ID (NCBI): 144699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572).

Publikationen

Species	Application	Title
human, mouse	WB	Cell Death Dis; F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7.; Authors - Y Liu
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes