ERC1 Polyklonaler Antikörper

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• KD/KO Validated

ERC1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 22211-1-AP

Synonyme

Cast2, ELKS, ERC 1, ERC1, KIAA1081, Rab6 interacting protein 2, RAB6IP2

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 22211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22211-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22211-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22211-1-AP (ERC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 22211-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ERC1 antibody 22211-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, humanes Hodengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

22211-1-AP bindet in WB, IF, CoIP, ELISA ERC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERC1 fusion protein Ag17536
• Vollständiger Name: ELKS/RAB6-interacting/CAST family member 1
• Berechnetes Molekulargewicht: 1116 aa, 128 kDa
• Beobachtetes Molekulargewicht: 124-135 kDa
• GenBank-Zugangsnummer: BC132782
• Gene symbol: ERC1
• Gene ID (NCBI): 23085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).

Publikationen

Species	Application	Title
	WB	Sci Rep; Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry.; Authors - Qingyang Liu
rat	WB,CoIP	J Cell Sci; Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells.; Authors - Ivar Noordstra
human	WB	J Biol Chem; MICAL3 flavoprotein monooxygenase forms a complex with centralspindlin and regulates cytokinesis.; Authors - Qingyang Liu ; KD Validated
human	IF	J Cell Biol; RAB6 and microtubules restrict protein secretion to focal adhesions.; Authors - Lou Fourriere

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Ab performs well by Immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200 IF
• Cell Tissue Type: HUVEC

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