EMILIN1 Monoklonaler Antikörper

EMILIN1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2A5C8

Kat-Nr. : 60047-1-Ig

Synonyme

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

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Galerie der Validierungsdaten

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  WB analysis of human colon using 60047-1-Ig

  Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of human rectum using 60047-1-Ig

  human rectum tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 60047-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human ovary tumor using 60047-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human ovary tumor using 60047-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, humanes Rektumgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolongewebe, humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Ovarialkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60047-1-Ig bindet in WB, IHC, IF, ELISA EMILIN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EMILIN1 fusion protein Ag1016
• Vollständiger Name: elastin microfibril interfacer 1
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC007530
• Gene symbol: EMILIN1
• Gene ID (NCBI): 11117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Publikationen

Species	Application	Title
human	IF	Connect Tissue Res; EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro.; Authors - Nakatomi Yuka Y
human	IHC	Hernia; Elastic fibers in the anterior abdominal wall.; Authors - Fachinelli A A

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