ELF2 Polyklonaler Antikörper

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• KD/KO Validated

ELF2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12499-1-AP

Synonyme

E74 like factor 2, ELF2, ELF2A, EU32, NERF, NERF 1A, NERF 1a,b, NERF 1B, NERF 2, New ETS related factor

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 12499-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12499-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12499-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 12499-1-AP

  IP result of anti-ELF2 (IP:12499-1-AP, 4ug; Detection:12499-1-AP 1:500) with A2780 cells lysate 960ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, humanes Hirngewebe, humanes Lebergewebe
• Erfolgreiche IP: A2780-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

12499-1-AP bindet in WB, IP, ELISA ELF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELF2 fusion protein Ag3170
• Vollständiger Name: E74-like factor 2 (ets domain transcription factor)
• Berechnetes Molekulargewicht: 533 aa, 57 kDa
• Beobachtetes Molekulargewicht: 63-64 kDa, 56-57 kDa
• GenBank-Zugangsnummer: BC034951
• Gene symbol: ELF2
• Gene ID (NCBI): 1998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.

Publikationen

Species	Application	Title
goat	WB	Front Cell Dev Biol; A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.; Authors - Yue Zhang ; KD Validated
human	WB	Cell Biol Int; Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.; Authors - Yunpeng Bai
mouse	WB	Ren Fail; ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.; Authors - Shizhu Yuan