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  • KD/KO Validated

Dystrophin Polyklonaler Antikörper

Dystrophin Polyklonal Antikörper für WB, IHC, IF, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12715-1-AP

Synonyme

DMD, BMD



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, Maushirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaus-Skelettmuskelgewebe, Rattenherzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12715-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA Dystrophin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Dystrophin fusion protein Ag3408
Vollständiger Name dystrophin
Berechnetes Molekulargewicht 3685 aa, 427 kDa
Beobachtetes Molekulargewicht70 kDa, 430 kDa
GenBank-ZugangsnummerBC028720
Gene symbol Dystrophin
Gene ID (NCBI) 1756
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Protokolle

Produktspezifische Protokolle
WB protocol for Dystrophin antibody 12715-1-APProtokoll herunterladen
IHC protocol for Dystrophin antibody 12715-1-APProtokoll herunterladen
IF protocol for Dystrophin antibody 12715-1-APProtokoll herunterladen
IP protocol for Dystrophin antibody 12715-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Biomed Eng

Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.

Authors - Ningning Shi
mouseWB,IF

Sci Adv

Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders

Authors - Jihad El Andari
mouseWB,IF

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
  • KD Validated
mouseIF

Oxid Med Cell Longev

Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.

Authors - Wei Zou
ratIHC

Oxid Med Cell Longev

Knockdown of Salusin-β Improves Cardiovascular Function in Myocardial Infarction-Induced Chronic Heart Failure Rats.

Authors - Yu Xu
mouseIF

Nanomedicine

Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination

Authors - JingWen Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lauren (Verified Customer) (08-10-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: stem cell-derived myotubes
Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)