DEK Polyklonaler Antikörper

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• KD/KO Validated

DEK Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 16448-1-AP

Synonyme

D6S231E, DEK, DEK oncogene, Protein DEK

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Galerie der Validierungsdaten

• 

  WB analysis using 16448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16448-1-AP

  IP Result of anti-DEK (IP:16448-1-AP, 4ug; Detection:16448-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of mouse brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe, T-47D-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Produktinformation

16448-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA DEK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DEK fusion protein Ag8225
• Vollständiger Name: DEK oncogene
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-50 kDa
• GenBank-Zugangsnummer: BC035259
• Gene symbol: DEK
• Gene ID (NCBI): 7913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transduction. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Publikationen

Species	Application	Title
mouse	IF	Nature; Maintenance of muscle stem-cell quiescence by microRNA-489.; Authors - Cheung Tom H TH
mouse	IF	Dev Cell; Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.; Authors - Chih-Jen Lin
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Dev Cell; Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit.; Authors - Lu Yue
mouse	WB	Oncogene; Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.; Authors - Y Patel
human,mouse	WB,IF,ChIP	Oncogene; Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.; Authors - Yao-Shun Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well by IF on PC3 cells fixed in 4% PFA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: PC3 cells

DEK Antibody Immunofluorescence validation (1:25 dilution) in PC3 cells (Cat no:16448-1-AP)

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