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CRTC2,TORC2 Polyklonaler Antikörper

CRTC2,TORC2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12497-1-AP

Synonyme

CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, HEK-293-Zellen, Mauslebergewebe
Erfolgreiche Detektion in IHChumanes Hautkrebsgewebe, humanes Gliomgewebe, humanes Urothelkarzinomgewebe, Mauslungengewebe, Mausnierengewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12497-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CRTC2,TORC2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CRTC2,TORC2 fusion protein Ag3167
Vollständiger Name CREB regulated transcription coactivator 2
Berechnetes Molekulargewicht 693 aa, 73 kDa
Beobachtetes Molekulargewicht 73 kDa
GenBank-ZugangsnummerBC053562
Gene symbol TORC2
Gene ID (NCBI) 200186
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Protokolle

Produktspezifische Protokolle
WB protocol for CRTC2,TORC2 antibody 12497-1-APProtokoll herunterladen
IHC protocol for CRTC2,TORC2 antibody 12497-1-APProtokoll herunterladen
IF protocol for CRTC2,TORC2 antibody 12497-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

Adv Sci (Weinh)

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Authors - Zeyun Mi
  • KD Validated
mouseWB

Mol Cell

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation

Authors - Shelby E Compton
humanIHC

Am J Transplant

The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.

Authors - Qi Ling
mouseIHC,IF

J Neurosci

Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.

Authors - Sakamoto Kensuke K
human,mouse,bovineWB,IF

J Neurosci

CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.

Authors - Hideaki Kanki
humanWB,IF

J Virol

Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.

Authors - Yuan Jinxiang J