COPE Polyklonaler Antikörper

COPE Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11457-1-AP

Synonyme

Epsilon-COP, Epsilon-coat protein, Coatomer subunit epsilon

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11457-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11457-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11457-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11457-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11457-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11457-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11457-1-AP (COPE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11457-1-AP

  Immunofluorescent analysis of HepG2 cells, using COPE antibody 11457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, A431-Zellen, HeLa-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11457-1-AP bindet in WB, IHC, IF/ICC, ELISA COPE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPE fusion protein Ag2024
• Vollständiger Name: coatomer protein complex, subunit epsilon
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC017285
• Gene symbol: COPE
• Gene ID (NCBI): 11316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

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