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CLN3 Polyklonaler Antikörper

CLN3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20386-1-AP

Synonyme

Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of SH-SY5Y cells using CLN3 Polyclonal antibody (20386-1-AP)

    WB analysis of SH-SY5Y using 20386-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20386-1-AP (CLN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CLN3 Polyclonal antibody (20386-1-AP)

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CLN3 Polyclonal antibody (20386-1-AP)

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLN3 Polyclonal antibody (20386-1-AP)

    IF Staining of HepG2 using 20386-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLN3 antibody 20386-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen
Erfolgreiche Detektion in IHChumanes Hirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20386-1-AP bindet in WB, IHC, IF, ELISA CLN3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CLN3 fusion protein Ag14224
Vollständiger Name ceroid-lipofuscinosis, neuronal 3
Berechnetes Molekulargewicht 438 aa, 48 kDa
Beobachtetes Molekulargewicht 45-48 kDa
GenBank-ZugangsnummerBC002394
Gene symbol CLN3
Gene ID (NCBI) 1201
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).

Protokolle

Produktspezifische Protokolle
WB protocol for CLN3 antibody 20386-1-APProtokoll herunterladen
IHC protocol for CLN3 antibody 20386-1-APProtokoll herunterladen
IF protocol for CLN3 antibody 20386-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen