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CLEC1A Polyklonaler Antikörper

CLEC1A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13394-1-AP

Synonyme

C type lectin like receptor 1, CLEC 1, CLEC1, CLEC1A

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CLEC1A Polyclonal antibody (13394-1-AP)

    WB analysis using 13394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13394-1-AP (CLEC1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    WB analysis of mouse thymus using 13394-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13394-1-AP (CLEC1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of human lung cancer using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13394-1-AP (CLEC1A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of human lung cancer using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13394-1-AP (CLEC1A antibody) at dilution of 1:100 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslungengewebe, Maus-Thymusgewebe, Rattenlungengewebe
Erfolgreiche Detektion in IHCMauslungengewebe, humanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

13394-1-AP bindet in WB, IF, IHC, ELISA CLEC1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CLEC1A fusion protein Ag4058
Vollständiger Name C-type lectin domain family 1, member A
Berechnetes Molekulargewicht 280 aa, 32 kDa
Beobachtetes Molekulargewicht 50 kDa, 32 kDa
GenBank-ZugangsnummerBC039072
Gene symbol CLEC1A
Gene ID (NCBI) 51267
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLEC1A (C-type lectin domain family 1 member A) is a single-pass type II membrane protein that contains one C-type lectin domain. Members of the C-type lectin domain family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC1A is expressed preferentially in dendritic cells and plays a role in regulating dendritic cell function (PMID: 10671229).

Protokolle

Produktspezifische Protokolle
WB protocol for CLEC1A antibody 13394-1-APProtokoll herunterladen
IHC protocol for CLEC1A antibody 13394-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanIF

Invest Ophthalmol Vis Sci

CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.

Authors - Guoqiang Zhu
View Article