CLCNKA Polyklonaler Antikörper

CLCNKA Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14402-1-AP

Synonyme

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

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Galerie der Validierungsdaten

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  WB analysis using 14402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14402-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14402-1-AP

  IP Result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Nierengewebe, Mausnierengewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14402-1-AP bindet in WB, IP, IHC, ELISA CLCNKA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLCNKA fusion protein Ag5647
• Vollständiger Name: chloride channel Ka
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC048282
• Gene symbol: CLCNKA
• Gene ID (NCBI): 1187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

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