CIAPIN1 Polyklonaler Antikörper

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• KD/KO Validated

CIAPIN1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12638-1-AP

Synonyme

PRO0915, Fe-S cluster assembly protein DRE2 homolog, DRE2, Cytokine-induced apoptosis inhibitor 1, CUA001

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12638-1-AP

  WB result of CIAPIN1 antibody (12638-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CIAPIN1 transfected HeLa cells.

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  WB analysis using 12638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12638-1-AP (CIAPIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 12638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12638-1-AP (CIAPIN1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12638-1-AP (CIAPIN1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12638-1-AP

  Immunofluorescent analysis of HeLa cells using 12638-1-AP (CIAPIN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

12638-1-AP bindet in WB, IHC, IF/ICC, ELISA CIAPIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIAPIN1 fusion protein Ag3333
• Vollständiger Name: cytokine induced apoptosis inhibitor 1
• Berechnetes Molekulargewicht: 312 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC024196
• Gene symbol: CIAPIN1
• Gene ID (NCBI): 57019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cytokine-induced anti-apoptotic molecule (CIAPIN1, or DRE2) had been found to be a differentially-expressed gene involved in a variety of cancers, and it was also considered as a candidate tumor suppressor gene in gastric cancer, renal cancer and liver cancer. CIAPIN1 plays an important role in the differentiation of colorectal cancer (CRC) cells, and the differential expression of CIAPIN1 in CRC was closely related to prognosis. CIAPIN1 might play an important role in esophageal carcinogenesis, and it could be considered as a valuable prognostic indicator in esophageal squamous cell carcinoma (ESCC).

Publikationen

Species	Application	Title
human	WB	Hum Mol Genet; Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.; Authors - Ki Soon Kim
rat	WB	ACS Chem Neurosci; Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity; Authors - Xinglai Zhang
mouse	WB	Front Oncol; Downregulation of miR-142a Contributes to the Enhanced Anti-Apoptotic Ability of Murine Chronic Myelogenous Leukemia Cells.; Authors - Zhiwei Chen
human	WB	Sci Adv; Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer; Authors - Subhadip Mukhopadhyay
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio