CDK6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDK6 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

156

Kat-Nr. : 14052-1-AP

Synonyme

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE

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Galerie der Validierungsdaten

• 

  WB analysis using 14052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14052-1-AP

  WB result of CDK6 antibody (14052-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

• 

  WB analysis of MOLT-4 using 14052-1-AP

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 cells using 14052-1-AP

  C2C12 cells (shRNA samples) were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:500. .

• 

  IP experiment of Jurkat using 14052-1-AP

  IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:1000) with Jurkat cells lysate 2880 ug.

• 

  IHC staining of human lung cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14052-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14052-1-AP (CDK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14052-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (14052-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C2C12-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Urothelkarzinomgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 148 publications below
• IHC: See 15 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14052-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA CDK6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK6 fusion protein Ag5275
• Vollständiger Name: cyclin-dependent kinase 6
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC027989
• Gene symbol: CDK6
• Gene ID (NCBI): 1021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a 

protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition, 

thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to 

release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication 

(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell 

cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions, 

where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.

What is the molecular weight of CDK6?

The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).

What is the subcellular localization of CDK6?

CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are 

located in the nuclei of proliferating cells.

What is the expression pattern of CDK6?

CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and 

members of the INK4 family of CDK inhibitors (PMID: 7739547).

What is CDK6's role in cancer?

CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately 

80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the 

hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation 

of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6 

expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J
human	WB,IHC	Nat Commun; The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer; Authors - Wei Zhou
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	Cancer Res; CDK4/6 inhibition enhances oncolytic virus efficacy by potentiating tumor-selective cell killing and T cell activation in refractory glioblastoma.; Authors - Deyin Guo ; KD Validated
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song

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