CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

3H5

Kat-Nr. : FITC-65217

Synonyme

B7 1, CD80, Cd80 molecule

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Galerie der Validierungsdaten

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  FC experiment of wistar rat splenocytes using FITC-65217

  1X10^6 unstimulated (dashed) or LPS-stimulated Wistar rat splenocytes were surface stained with 0.5 ug FITC Plus Anti-Rat CD80 (FITC-65217, Clone: 3H5) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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  FC experiment of wistar rat splenocytes using FITC-65217

  1X10^6 unstimulated (lower) or LPS-stimulated Wistar rat splenocytes (upper) were surface stained with 0.5 ug FITC Plus Anti-Rat CD80 (FITC-65217, Clone:3H5) (red) or 0.5 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS-stimulated Wistar rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65217 bindet in FC CD80 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Cd80 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: XM_017597885
• Gene symbol: Cd80
• Gene ID (NCBI): 25408
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)