Anti-Rat CD80/B7-1 (3H5) Mouse IgG2a Recombinant Antibody

CD80/B7-1 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

3H5

Cat no : 65580-1-MR

Synonyms

CD80, Cd80 molecule, B7-1, B7.1, B7 1

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Validation Data Gallery

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  FC experiment of rat splenocytes using 65580-1-MR

  1x10^6 unstimulated or LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Rat CD45RA (OX-33). Cells were not fixed.

• 

  FC experiment of rat splenocytes using 65580-1-MR

  1x10^6 LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Tested Applications

• Positive FC detected in: LPS-stimulated rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65580-1-MR targets CD80/B7-1 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Full Name: Cd80 molecule
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: XM_017597885
• Gene Symbol: Cd80
• Gene ID (NCBI): 25408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)