CD46 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD46 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 12494-1-AP

Synonyme

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

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Galerie der Validierungsdaten

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  WB analysis using 12494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12494-1-AP (CD46 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12494-1-AP

  IP result of anti-CD46 (IP:12494-1-AP, 4ug; Detection:12494-1-AP 1:1000) with A549 cells lysate 1800ug.

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12494-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD46 antibody (12494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• FC: See 1 publications below

Produktinformation

12494-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CD46 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD46 fusion protein Ag3131
• Vollständiger Name: CD46 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 384 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC030594
• Gene symbol: CD46
• Gene ID (NCBI): 4179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

Publikationen

Species	Application	Title
human	WB, FC	Mol Immunol; Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.; Authors - Frida C Mohlin
human	WB	Oncol Rep; Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.; Authors - Zejun Lu ; KD Validated
mouse	WB	Clin Exp Immunol; Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.; Authors - Yingchun Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (04-12-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

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