CCR2a-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CCR2a-specific Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (3)

Kat-Nr. : 16153-1-AP

Synonyme

C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R

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Galerie der Validierungsdaten

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  WB analysis using 16153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16153-1-AP (CCR2a-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16153-1-AP

  WB result of CCR2a-specific antibody (16153-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCR2a-specific transfected Jurkat cells.

• 

  IHC staining of human kidney using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 16153-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 16153-1-AP (CCR2-specific antibody at dilution of 1:50.

• 

  FC experiment of Jurkat using 16153-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug CCR2-specific antibody (16153-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

16153-1-AP bindet in WB, IHC, IF, FC, ELISA CCR2a-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: chemokine (C-C motif) receptor 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_000647
• Gene symbol: CCR2
• Gene ID (NCBI): 729230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.

Publikationen

Species	Application	Title
human	WB	Lab Invest; Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.; Authors - Shin Ung Kang
human	WB	Oncol Rep; CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.; Authors - Huijie Zhuang
rat	WB	J Ethnopharmacol; Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis; Authors - Tian Xu
mouse	WB,IF	Mol Immunol; QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.; Authors - Lele Deng
human,rat	WB,IHC	J Physiol Biochem; Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues; Authors - Bairong Fang
human	IHC	Clin Exp Rheumatol; Curcumin alleviates inflammation in Takayasu's arteritis by blocking CCL2 overexpression in adventitial fibroblasts.; Authors - Sifan Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal Baral (Verified Customer) (01-26-2024)

Bone marrow mesenchymal stem cells from mouse stained great at the provided dilution.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse bone marrow

FH

Kamal (Verified Customer) (01-26-2024)

Bands appeared at 35 kDa. Antibody works well when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

Alexandru (Verified Customer) (11-13-2023)

Great stainig results on paraffin-embedded tissue!

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

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