MCP-1/CCL2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Mcp1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (1)

Applications

WB, IHC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

133

CloneNo.

1B9F7

Cat no : 66272-1-Ig

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

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Validation Data Gallery

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  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66272-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: DC2.4 cells, LPS treated RAW 264.7 cells, mouse brain tissue, mouse lung tissue, mouse kidney tissue, mouse placenta tissue
• Positive IHC detected in: mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 97 publications below
• IHC: See 33 publications below
• IF: See 18 publications below
• CoIP: See 1 publications below

Product Information

66272-1-Ig targets Mcp1 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mcp1 fusion protein Ag24085
• Full Name: chemokine (C-C motif) ligand 2
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• RRID: AB_2861337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publications

Species	Application	Title
	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
	IHC	Cell Rep Med; Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma; Authors - Junju Chen
mouse	IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li