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Mcp1 Monoclonal antibody

Mcp1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1B9F7

Cat no : 66272-1-Ig

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF



Tested Applications

Positive WB detected inDC2.4 cells, LPS treated RAW 264.7 cells, mouse brain tissue, mouse lung tissue, mouse kidney tissue, mouse placenta tissue
Positive IHC detected inmouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66272-1-Ig targets Mcp1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mcp1 fusion protein Ag24085
Full Name chemokine (C-C motif) ligand 2
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-011333
Gene Symbol Ccl2
Gene ID (NCBI) 20296
RRIDAB_2861337
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Protocols

Product Specific Protocols
WB protocol for Mcp1 antibody 66272-1-IgDownload protocol
IHC protocol for Mcp1 antibody 66272-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
mouseIF

Gut

Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.

Authors - Yunben Yang
mouseIF

Hepatology

Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.

Authors - Wu Yang
mouseIHC

Cell Death Differ

Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis

Authors - Qing-Xin Ji
mouseWB

Nat Commun

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Authors - Xinzhi Li
mouseWB

Nat Commun

Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response.

Authors - Ruhul Amin