CCR10 Polyklonaler Antikörper

CCR10 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22071-1-AP

Synonyme

C C chemokine receptor type 10, C C CKR 10, CC CKR 10, CCR 10, CCR10, G protein coupled receptor 2, GPR2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 22071-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22071-1-AP (CCR10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22071-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22071-1-AP (CCR10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 22071-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 22071-1-AP (CCR10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 22071-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 22071-1-AP (CCR10 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HepG2 using 22071-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CCR10 antibody (22071-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• FC: See 1 publications below

Produktinformation

22071-1-AP bindet in WB, IHC, FC, ELISA CCR10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCR10 fusion protein Ag17212
• Vollständiger Name: chemokine (C-C motif) receptor 10
• Berechnetes Molekulargewicht: 362 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC099840
• Gene symbol: CCR10
• Gene ID (NCBI): 2826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCR10 (also known as GPR2) is a G-protein coupled receptor for chemokines CCL27 (SCYA27) and CCL28 (SCYA28). CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (PMID: 11821900). CCR10-CCL28 interactions are thought to contribute to the accumulation of IgA Ab-secreting cells (ASC) to mucosal surfaces.

Publikationen

Species	Application	Title
mouse	WB,FC	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Front Immunol; Single-Cell Sequencing Reveals the Transcriptome and TCR Characteristics of pTregs and in vitro Expanded iTregs.; Authors - Zhenzhen Hui
human	IHC	Mol Med Rep; Knockdown of CCL28 inhibits endometriosis stromal cell proliferation and invasion via ERK signaling pathway inactivation; Authors - YINGTING WU
mouse	IHC	Inflamm Regen; Th22 is the effector cell of thymosin β15-induced hair regeneration in mice; Authors - Nana Tao

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