CTRP1 Polyklonaler Antikörper

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• KD/KO Validated

CTRP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12209-1-AP

Synonyme

C1QTNF1, UNQ310/PRO353, GIP, G protein-coupled receptor-interacting protein, Complement C1q tumor necrosis factor-related protein 1

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Galerie der Validierungsdaten

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  WB analysis of human testis using 12209-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12209-1-AP (CTRP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12209-1-AP

  Immunofluorescent analysis of HepG2 cells, using C1QTNF1 antibody 12209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

12209-1-AP bindet in WB, IHC, IF/ICC, ELISA CTRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTRP1 fusion protein Ag2854
• Vollständiger Name: C1q and tumor necrosis factor related protein 1
• Berechnetes Molekulargewicht: 281 aa, 32 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC021553
• Gene symbol: CTRP1
• Gene ID (NCBI): 114897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

Publikationen

Species	Application	Title
rat	WB	Front Cell Dev Biol; CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway.; Authors - Huizhi Fei ; KD Validated
human	WB	Biomed Res Int; Identification of CTRP1 as a Prognostic Biomarker and Oncogene in Human Glioblastoma.; Authors - Liyan Chen ; KD Validated
human	IHC	Front Mol Biosci; High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma; Authors - Jiechuan Qiu

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