VIPAS39 Polyklonaler Antikörper

VIPAS39 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20771-1-AP

Synonyme

C14orf133, hSPE 39, Protein spe 39 homolog, VIPAR, VIPAS39, VPS33B interacting protein

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Galerie der Validierungsdaten

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  WB analysis using 20771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20771-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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  IF Staining of HepG2 using 20771-1-AP

  Immunofluorescent analysis of HepG2 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HEK-293 using 20771-1-AP

  Immunofluorescent analysis of 293 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, humanes Nierengewebe, L02-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20771-1-AP bindet in WB, IHC, IF, ELISA VIPAS39 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VIPAS39 fusion protein Ag14762
• Vollständiger Name: chromosome 14 open reading frame 133
• Berechnetes Molekulargewicht: 493 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC015054
• Gene symbol: C14orf133
• Gene ID (NCBI): 63894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; FERARI is required for Rab11-dependent endocytic recycling.; Authors - Jachen A Solinger
human	WB	Virus Genes; Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.; Authors - Ye Li

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