ATP1A3 (C-terminal) Polyklonaler Antikörper

ATP1A3 (C-terminal) Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10868-1-AP

Synonyme

ATP1A3, ATP1A3 (C-terminal), DYT12, RDP, Sodium pump subunit alpha 3

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Galerie der Validierungsdaten

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  WB analysis of C2C12 using 10868-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12 cell, C2C12-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Cerebellum-Gewebe, Maushirngewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

10868-1-AP bindet in WB, IHC, IF, ELISA ATP1A3 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A3 (C-terminal) fusion protein Ag1313
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-113 kDa
• GenBank-Zugangsnummer: BC015566
• Gene symbol: ATP1A3
• Gene ID (NCBI): 478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na⁺/K⁺ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Publikationen

Species	Application	Title
human	WB	Front Oncol; Elevated Sodium Pump α3 Subunit Expression Promotes Colorectal Liver Metastasis via the p53-PTEN/IGFBP3-AKT-mTOR Axis.; Authors - Di Wu
human	IHC	Cancer Cell Int; Comprehensive analysis of the expression of sodium/potassium-ATPase α subunits and prognosis of ovarian serous cystadenocarcinoma.; Authors - Wei Huang
human	WB,IHC	Life Sci; Identification of a robust scoring system based on metabolic genes followed by in-depth validation of ATP1A3 in glioma; Authors - Qiankun Ji