• Überblick
  • Geprüfte Anwendungen
  • Produktinformation
  • Publikationen (12)
  • Protokolle
  • Verwandte Produkte (5)
  • Featured Product
  • KD/KO Validated

5 Lipoxygenase Polyklonaler Antikörper

5 Lipoxygenase Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10021-1-Ig

Synonyme

ALOX5, 5LOX, 5-LO, 5LO, 5-lipoxygenase



Geprüfte Anwendungen

Erfolgreiche Detektion in WBDU 145-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, PC-3-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10021-1-Ig bindet in WB, IHC, IF/ICC, ELISA 5 Lipoxygenase und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name arachidonate 5-lipoxygenase
Berechnetes Molekulargewicht 674 aa, 78 kDa
Beobachtetes Molekulargewicht 70-78 kDa
GenBank-ZugangsnummerBC130332
Gene symbol 5 Lipoxygenase
Gene ID (NCBI) 240
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Protokolle

Produktspezifische Protokolle
WB protocol for 5 Lipoxygenase antibody 10021-1-IgProtokoll herunterladen
IF protocol for 5 Lipoxygenase antibody 10021-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
mouseWB,IHC

Front Pharmacol

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway

Authors - Tong Zhou
human,mouseWB,IHC,IF

Int Immunopharmacol

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis

Authors - Wei-Min Hu
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
humanWB

J Biol Chem

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

Authors - Sivalokanathan Sarveswaran
  • KD Validated
humanWB

PLoS One

MK591, a Second Generation Leukotriene Biosynthesis Inhibitor, Prevents Invasion and Induces Apoptosis in the Bone-Invading C4-2B Human Prostate Cancer Cells: Implications for the Treatment of Castration-Resistant, Bone-Metastatic Prostate Cancer.

Authors - Sivalokanathan Sarveswaran
ratIHC

Exp Cell Res

Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Authors - Fen Xu

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