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ADRP/Perilipin 2 Polyklonaler Antikörper
ADRP/Perilipin 2 Polyklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15294-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Mauslebergewebe, K-562-Zellen, NIH/3T3-Zellen, Rattenlebergewebe |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Nierenzellkarzinomgewebe, humanes Prostatakarzinomgewebe, Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | mit Ölsäure behandelte HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunhistochemie (IHC) | IHC : 1:200-1:8000 |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 67 publications below |
IHC | See 20 publications below |
IF | See 38 publications below |
Produktinformation
15294-1-AP bindet in WB, IHC, IF, ELISA ADRP/Perilipin 2 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Hund, Maus, Ratte, Rind, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | ADRP/Perilipin 2 fusion protein Ag7539 |
Vollständiger Name | adipose differentiation-related protein |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 45-48 kDa |
GenBank-Zugangsnummer | BC005127 |
Gene symbol | ADFP |
Gene ID (NCBI) | 123 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Protokoll herunterladen |
IHC protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Protokoll herunterladen |
IF protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nature iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Commun Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy | ||
Mol Cell Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation. | ||
Nat Commun O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Liliana (Verified Customer) (02-13-2024) | WORKS VERY WELL ON PH9, 1:2000 AND ON PH6, 1:1000
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FH Christine (Verified Customer) (09-19-2023) | Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).
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FH Kamal (Verified Customer) (07-05-2023) | Worked well when diluted with 1X PBS.
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FH Christin (Verified Customer) (03-09-2023) | Great antibody for WB with low background signal.
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FH Nick (Verified Customer) (04-20-2022) | Helped localize expression of Plin2
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FH Boyan (Verified Customer) (05-09-2019) | Very good for WB
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