6*His, His-tag Polyklonaler Antikörper

6*His, His-tag Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (5)

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

75

Rezensionen (2)

Kat-Nr. : 10001-0-AP

Synonyme

6*his,His tag, 6*His, 6 his, his tag, his

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis of recombinant protein using 10001-0-AP

  Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.

"6*His, His-tag Antibodies" Comparison

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Geprüfte Anwendungen

Veröffentlichte Anwendungen

• WB: See 53 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 6 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

10001-0-AP bindet in WB, IHC, IF, IP, ChIP, ELISA 6*His, His-tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: 6*His, His-tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.

Publikationen

Species	Application	Title
	ChIP	Nature; High-resolution landscape of an antibiotic binding site; Authors - Kevin B Yang
	WB	Am J Respir Crit Care Med; Targeting Procalcitonin Protects Vascular Barrier Integrity; Authors - Laura Brabenec
	WB	Nat Commun; USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.; Authors - Shangda Yang
human		Cell Death Differ; SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress; Authors - Jun Luo
	WB	Nat Commun; Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.; Authors - Jun Zhou
		Nat Commun; Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome.; Authors - Lianhui Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brogan (Verified Customer) (11-09-2022)

Works very well, will buy again

• Applications: Western Blot, Other

FH

Jean-Baptiste (Verified Customer) (04-21-2022)

Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

6*His, His-tag Antibody Western Blot validation (1:2000 dilution) in (Cat no:10001-0-AP)