• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WFS1 Monoclonal antibody

WFS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C2B5

Cat no : 67291-1-Ig

Synonyms

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A172 cells using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis of A172 using 67291-1-Ig

    A172 cells were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using WFS1 Monoclonal antibody (67291-1-Ig)

    WB analysis using 67291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, A172 cells, HT-29 cells, HEK-293 cells, C6 cells, U-251 cells, PC-12 cells, Neuro-2a cells, SW480 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67291-1-Ig targets WFS1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WFS1 fusion protein Ag25735
Full Name Wolfram syndrome 1 (wolframin)
Calculated Molecular Weight 890 aa, 100 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC030130
Gene Symbol WFS1
Gene ID (NCBI) 7466
RRIDAB_2882556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Protocols

Product Specific Protocols
WB protocol for WFS1 antibody 67291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols