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Tested Applications
| Positive WB detected in | rat lung tissue |
| Positive IHC detected in | human lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
26909-1-AP targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Uteroglobin/CC10 fusion protein Ag25530 Predict reactive species |
| Full Name | secretoglobin, family 1A, member 1 (uteroglobin) |
| Calculated Molecular Weight | 10 kDa |
| Observed Molecular Weight | 9 kDa |
| GenBank Accession Number | BC004481 |
| Gene Symbol | Uteroglobin |
| Gene ID (NCBI) | 7356 |
| RRID | AB_2880680 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Uteroglobin/CC10 antibody 26909-1-AP | Download protocol |
| IHC protocol for Uteroglobin/CC10 antibody 26909-1-AP | Download protocol |
| IF protocol for Uteroglobin/CC10 antibody 26909-1-AP | Download protocol |
| FC protocol for Uteroglobin/CC10 antibody 26909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Regulation of MHC Class I Expression in Lung Epithelial Cells during Inflammation. | ||
Front Pediatr Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia. | ||
Front Cell Infect Microbiol BPIFA1 is a secreted biomarker of differentiating human airway epithelium |
