UGT2B7 Polyclonal antibody

UGT2B7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16661-1-AP

Synonyms

UDPGT 2B9, UDPGT 2B7, UDP-glucuronosyltransferase 2B9, UDP-glucuronosyltransferase 2B7, EC:2.4.1.17



Tested Applications

Positive WB detected inrat liver tissue, HEK-293 cells
Positive IP detected inrat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16661-1-AP targets UGT2B7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UGT2B7 fusion protein Ag10121
Full Name UDP glucuronosyltransferase 2 family, polypeptide B7
Calculated Molecular Weight 529 aa, 61 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC030974
Gene Symbol UGT2B7
Gene ID (NCBI) 7364
RRIDAB_2214249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UGT2B7 antibody 16661-1-APDownload protocol
IHC protocol for UGT2B7 antibody 16661-1-APDownload protocol
IF protocol for UGT2B7 antibody 16661-1-APDownload protocol
IP protocol for UGT2B7 antibody 16661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Divergent Expression and Metabolic Functions of Human Glucuronosyltransferases through Alternative Splicing.

Authors - Michèle Rouleau
humanWB

Br J Pharmacol

UDP-Glucuronosyltransferase (UGT)-Mediated Attenuations of Cytochrome P450 3A4 Activity: UGT Isoform-Dependent Mechanism of Suppression.

Authors - Yuu Miyauchi
WB

Sci Rep

Inter-isoform Hetero-dimerization of Human UDP-Glucuronosyltransferases (UGTs) 1A1, 1A9, and 2B7 and Impacts on Glucuronidation Activity.

Authors - Ling-Min Yuan
humanWB

Sci Rep

Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.

Authors - Yanyan Gao
WB

Biochem Pharmacol

PMID: 25770680

Authors - Lingmin Yuan
WB

Front Pharmacol

Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4.

Authors - Yuu Miyauchi