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TYRO3 Polyclonal antibody

TYRO3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28513-1-AP

Synonyms

Brt, BYK, Dtk, FLJ16467, RSE, Sky, Tif, TYRO3, TYRO3 protein tyrosine kinase, Tyrosine protein kinase byk, Tyrosine protein kinase DTK, Tyrosine protein kinase RSE, Tyrosine protein kinase SKY

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TYRO3 Polyclonal antibody (28513-1-AP)

    WB analysis using 28513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of human colon cancer using 28513-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of human colon cancer using 28513-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of mouse brain using 28513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of mouse brain using 28513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28513-1-AP targets TYRO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TYRO3 fusion protein Ag29540
Full Name TYRO3 protein tyrosine kinase
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC049368
Gene Symbol TYRO3
Gene ID (NCBI) 7301
RRIDAB_2881162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)

Protocols

Product Specific Protocols
WB protocol for TYRO3 antibody 28513-1-APDownload protocol
IHC protocol for TYRO3 antibody 28513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,FC

Cells

TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway

Authors - Quang Duy Trinh
View Article
humanIHC

Eur Arch Otorhinolaryngol

Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps

Authors - Yandan Zhou
View Article