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TYK2 Monoclonal antibody

TYK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1A10

Cat no : 67411-1-Ig

Synonyms

JTK1, TYK2, tyrosine kinase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TYK2 Monoclonal antibody (67411-1-Ig)

    WB analysis using 67411-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using TYK2 Monoclonal antibody (67411-1-Ig)

    WB analysis using 67411-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TYK2 Monoclonal antibody (67411-1-Ig)

    WB analysis using 67411-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human colon cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human colon cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Monoclonal antibody (67411-1-Ig)

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TYK2 Monoclonal antibody (67411-1-Ig)

    IF Staining of HepG2 using 67411-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (67411-1-Ig, Clone: 1E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, MJ cells, Jurkat cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, A549 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67411-1-Ig targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TYK2 fusion protein Ag9442
Full Name tyrosine kinase 2
Calculated Molecular Weight 1187 aa, 134 kDa
Observed Molecular Weight 134 kDa
GenBank Accession NumberBC014243
Gene Symbol TYK2
Gene ID (NCBI) 7297
RRIDAB_2882652
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TYK2 antibody 67411-1-IgDownload protocol
IHC protocol for TYK2 antibody 67411-1-IgDownload protocol
IF protocol for TYK2 antibody 67411-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

Invest New Drugs

IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4.

Authors - Jia Huo
View Article
humanWB

bioRxiv

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

Cancer Lett

HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma

Authors - Xueshuo Sun
View Article
mouseWB

Antioxidants (Basel)

Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses

Authors - Shuni Duan
View Article
mouseWB

Chin J Nat Med

Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses

Authors - Kexin Jia
View Article